Swab Collection and DNA Preservation System For collection, ambient storage and transport of DNA from samples collected using a swab For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Swab Collection and DNA Preservation System For collection, ambient storage and transport of DNA from samples collected using a swab USD$345.00 SKU 45690-B Size 50 Units Contact Distributor Overview Details Documentation Citations Overview Collect from nasal, buccal, saliva, fecal, skin, surfaces, and moreCompletely stabilize microbiota profiles from the point-of-collectionMinimize bias in data analysis: NGS (Metagenomics/Microbiome), PCR and microarraysNo need to immediately process samples: ship and store at ambient temperaturesDNA preservation at room temperature over 2 yearsRenders sample non-infectious for safe and easy shippingCompatible with DNA isolation kits and protocols: Optimized isolation procedure using Norgen's Microbiome DNA Isolation Kit Norgen’s Swab Collection and DNA Preservation System is designed for collection, ambient storage and transport of DNA from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Swab Collection and DNA Preservation System contains Norgen’s Swab Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Swab Preservative. The Swab Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Swab Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Swab Preservative allow DNA samples to be stored at room temperature for over 2 years.DNA Isolation from PreservativeSwab samples collected and preserved using Norgen’s Swab Collection and DNA Preservation System are compatible with most DNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA isolation kits and reagents. An optimized isolation procedure is available as part of Norgen’s Microbiome DNA Isolation Kit (Cat. 64100). Details Supporting Data Previous Figure 1. Stability of DNA Preserved in Norgen's Swab DNA Preservative at Room Temperature for up to 75 Months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Swab DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Swab DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100). Figure 2. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months. The quality of the saliva DNA stored at room temperature did not change from 1 week to 75 months, as indicated by the fact that the Ct values remained consistent. The improvement in the Ct values from the real-time PCR graph (red) also indicate the impact of the improved saliva DNA isolation method provided by the Saliva DNA Isolation Kit (Cat. RU45400). Figure 3. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points). All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition. Blue: Saliva DNA isolated using improved Saliva DNA Isolation Kit (Cat. RU45400). Black: No template control. Figure 4. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram. Next Click for expanded view Kit Specifications Volume of Preservative 1.2 mL Preservation Temperature Room Temperature Preservation time 2 years at room temperature Shelf Life and Handling When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date. Kit Components Cat. 45690 (50 units) Swab Collection and DNA Preservation Kit 50 Individually Wrapped Swabs 50 Product Insert 1 Documentation PROTOCOLS (45690-B) Swab Collection and DNA Preservation System - Protocol (50 units) SUPPLEMENTARY PROTOCOLS 45690 - Donor Procedure 45690 - Isolation of DNA from Norgen’s Swab Collection and DNA Preservation System using Norgen’s Saliva DNA Isolation Kit APPLICATION NOTES The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative SAFETY DATA SHEETS (45690-B) Swab Collection and DNA Preservation System - Safety Data Sheet POSTERS In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016 Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives FLYERS Swab Collection and DNA Preservation Customization Flyer Citations Powered by Bioz See more details on Bioz Related Products Microbiome DNA Isolation Kit Shipping Accessories Saliva DNA Isolation Kit Genomic DNA Isolation Kit Cells and Tissue DNA Isolation Kit
Swab Collection and DNA Preservation System For collection, ambient storage and transport of DNA from samples collected using a swab For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Swab Collection and DNA Preservation System For collection, ambient storage and transport of DNA from samples collected using a swab USD$345.00 SKU 45690-B Size 50 Units Contact Distributor