Saliva RNA Collection and Preservation Devices Dx Preserve High Quality RNA, including Viral RNA, for up to 2 Months Intended for in vitro diagnostics use. Powered by Bioz Saliva RNA Collection and Preservation Devices Dx Preserve High Quality RNA, including Viral RNA, for up to 2 Months SMART Save USD$629.00 USD$503.20 SKU 53800 Size 50 Devices Quantity Overview Details Documentation Citations Overview CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746. Ideal for use in in vitro diagnostic workflows Render samples non-infectious for safe shipping and handling Preserved RNA is suitable for a variety of downstream molecular applications, including PCR and qPCR Preserved RNA is stable for up to 2 months at room temperature Inactivates viruses including SARS-CoV-2 upon mixing - Explore the Application Note Non-invasive, user-friendly sample collection Suitable for self-collection of saliva samples supervised by a trained individual in the collection procedure by watching the training video and reading over the collection instructions. Norgen’s Saliva RNA Collection and Preservation Devices Dx are designed for 1) safe and non-invasive supervised self-collection of saliva specimens and 2) preservation of RNA in saliva specimens at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices Dx consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule. Saliva collection is performed through supervised self-collection. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits Dx (Cat# Dx69100, Dx69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for in vitro diagnostic use for medical purposes. This kit can be used to collect and preserve viral RNA, including SARs-CoV-2 RNA for detection of COVID-19. Norgen’s Saliva RNA Collection and Preservation Devices Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate these devices in conjunction with a downstream in vitro diagnostic assay. Saliva RNA Preservative Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh saliva specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature. The Saliva RNA Preservative also inactivates all bacteria, yeast and viruses present in the saliva sample. Nucleic Acid Isolation from Preservative Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits Dx (Cat# Dx69100, Dx69300). To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Details Supporting Data Previous Figure 1. Stability of RNA Preserved in Norgen's Saliva RNA Preservative at room temperature for up to 2 months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva RNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 2 months. Saliva RNA was subsequently isolated at day 0, day 4, day 6, day 16, 1 month and 2 months from 0.25 mL of the saliva/preservative sample using the supplementary protocol associated with Norgen's Total RNA Purification Kit (Cat. 17200). For visual analysis, 7.5 µL from 50 µL elution was mixed with RNA loading dye and was run on a 1x MOPS agarose gel. RNA integrity was maintained after the saliva samples were stored for up to 2 months at ambient temperatures in Norgen’s Saliva RNA Preservative. Figure 2. Stability of three house keeping genes over a 2 month period. GAPDH, Human 18S rRNA and beta actin genes were monitored from saliva samples stored at ambient temperature in Norgen's Saliva RNA Preservative. RNA was isolated from 250 µL of preserved samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgen's Total RNA Purification Kit (Cat. 17200). Next, 4 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad). All of the genes were preserved and stable over 2 month period. Figure 3. Norgen’s Saliva RNA Preservative is able to successfully preserve saliva RNA samples when used outside of the ideal range of 1:1 at room temperature for 3 days. As the amount of saliva added to 2 mL of Saliva RNA Preservative increased from 0.2 mL to 3 mL, RNase protein and β-actin gene were monitored for RNA stability using one-step RT-PCR. The results indicated that the variation of the saliva input volumes did not significantly influence the RNA gene detection. Figure 4. Detection of Viral, Bacterial and Human RNA from Preserved Saliva Samples. Saliva (2 mL) collected from a single healthy donor was mixed with 2 mL of Saliva RNA Preservative. Norgen’s COVID-19 positive control from the 2019-nCoV TaqMan RT-PCR Kit (E/RdRP gene) (Cat. TM67200) was spiked into the preserved saliva along with HeLa cells (1.6 m cells /ml ) to generate a contrived sample. From the contrived sample, total RNA was purified using Norgen’s Total RNA Purification Kit (Cat. 17200) and 5 genes (16s rRNA, COVID-19 E gene, RdRP-P1 and P2 gene and RP) were analyzed by PCR or RT-PCR to detect viral, bacterial and human genes. Next Click for expanded view Kit Specifications Volume of Saliva Collected 2 mL Volume of Saliva-Preservative Mix 4 mL Preservation Temperature Room Temperature Preservation time Up to 2 months at room temperature Shelf Life and Handling The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label). Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature. The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable. Kit ComponentsCat. RU53800 (50 Devices)Individual Saliva RNA Collection and Preservation Devices50Donor Procedure Flowchart1Product Insert1 Individual Saliva RNA Collection and Preservation Device Dx Contents Saliva Collection Funnel and Collection Tube1Collection Tube Cap1Preservative Ampoule1Donor Instructions1 Documentation PROTOCOLS (53800) Saliva RNA Collection and Preservation Devices Dx - Protocol (50 devices) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - Purification of Viral RNA with the Maxwell® RSC Viral Total Nucleic Acid Purification Kit APPLICATION NOTES Viral Inactivation Using Norgen’s Total Nucleic Acid Preservative and Norgen’s Saliva RNA Preservative Inactivation of Coronavirus in Samples using Norgen's Saliva RNA Preservative POSTERS Saliva Donor Flowchart FLYERS Customization Flyer Citations Powered by Bioz See more details on Bioz Related Products Saliva / Swab RNA Purification Kit Dx Total RNA Purification Kit Dx
Saliva RNA Collection and Preservation Devices Dx Preserve High Quality RNA, including Viral RNA, for up to 2 Months Intended for in vitro diagnostics use. Powered by Bioz Saliva RNA Collection and Preservation Devices Dx Preserve High Quality RNA, including Viral RNA, for up to 2 Months SMART Save USD$629.00 USD$503.20 SKU 53800 Size 50 Devices Quantity