Peach Latent Mosaic Viroid (PLMVd) TaqMan RT-PCR Kit Powered by Bioz Peach Latent Mosaic Viroid (PLMVd) TaqMan RT-PCR Kit SMART Save USD$749.00 USD$599.20 SKU TM64250 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for PLMVd Available in 1 format for analysis: Peach latent mosaic viroid (PLMVd) is a viroid specific to peach and consisting of a circular single-stranded RNA of 336-339 nt. It has been widely distributed around the world transmitted by graft propagation, and to a lesser extent by aphids. Although most infections occur without conspicuous symptoms, certain PLMVd isolates induce leaf mosaics, blotches and in the most extreme cases albinism (peach calico, PC), flower streaking, delays in foliation, flowering and ripening, deformations and decolorations of fruits, bud necrosis, stem pitting and premature ageing of the trees, which also adopt a characteristic growing pattern (open habit). PLMVd can be detected by molecular methods including polyacrylamide gel electrophoresis, dot-blot hybridization with radioactive and non-radioactive probes and RT-PCR. TaqMan PCR Kit Click to expand options Peach Latent Mosaic Viroid (PLMVd) TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Peach Latent Mosaic Viroid (PLMVd) TaqMan Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM64250 (100 preps) Cat. TM64210 (100 preps) MDx TaqMan 2X RT-PCR Master Mix 2 x 700 μL - PLMVd Primer & Probe Mix 280 μL 280 μL PLMVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS TM64200, TM64250 - Protocol (24 rxns, 100 rxns) TM64250 - Protocol (100 rxns) TM64210 - Protocol (100 rxns) SAFETY DATA SHEETS TM64200 - Safety Data Sheet
Peach Latent Mosaic Viroid (PLMVd) TaqMan RT-PCR Kit Powered by Bioz Peach Latent Mosaic Viroid (PLMVd) TaqMan RT-PCR Kit SMART Save USD$749.00 USD$599.20 SKU TM64250 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity