Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits SKU TM39750 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for HEV CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis The Enteroviruses are a genus of the family Picornaviridae, and are a large and diverse group of small RNA viruses characterized by a ssRNA molecule of positive sense. To date, over 70 serotypes of Enterovirus have been isolated from man. The human enteroviruses include poliovirus, Coxsackie A and Coxsackie B viruses, enterocytopathic human orphan (ECHO) viruses and other enteroviruses. Enteroviruses infect millions of people worldwide each year, and the infection can result in a wide variety of symptoms depending on the serotype of the enterovirus. Symptoms of infection may include, but are not limited to, mild respiratory illness (common cold), hand, foot and mouth disease, Rubelliform rashes, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, and neonatal infections. TaqMan PCR Kit Click to expand options HEV TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents HEV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM39750 (100 preps) Cat. TM39710 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Enterovirus Primer & Probe Mix 280 μL 280 μL Enterovirus Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM39750) Enterovirus TaqMan RT-PCR Kit - Protocol (100 rxns) SAFETY DATA SHEETS (TM39750) Enterovirus TaqMan RT-PCR Kit - Safety Data Sheet Related Products Human Enterovirus (HEV) TaqMan RT-PCR Kit Dx
Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits SKU TM39750 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity