Cryptosporidium TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cryptosporidium TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM39150 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Cryptosporidium CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection. TaqMan PCR Kit Click to expand options Cryptosporidium TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Cryptosporidium TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM39150 (100 preps) Cat. TM39110 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Cryptosporidium Primer & Probe Mix 280 μL 280 μL Cryptosporidium Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM39150) Cryptosporidium TaqMan RT-PCR Kit - Protocol (100 rxns) (TM39110) Cryptosporidium TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Cryptosporidium TaqMan RT-PCR Kit Dx Chrysanthemum Stunt Viroid (CSVd) TaqMan Probe/Primer and Control Set Water RNA/DNA Purification Kit - 0.22 µm
Cryptosporidium TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cryptosporidium TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM39150 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity