ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples USD$285.00 SKU 17300 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Rapid and efficient removal of abundant proteins from serum and plasma samples Process 10 samples in 30 minutes Allows for visualization of low abundance proteins Convenient and affordable spin column protocol Generic protocol allows for depletion from human and other animal samples Purification is based on spin column chromatography that uses Norgen’s resin separation matrix This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more. Details Supporting Data Previous Figure 1. Depletion of Abundant Proteins. Abundant proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. Lane 1 represents the input human serum proteins, while Lanes 3 and 4 represent the elution after the abundant proteins were depleted with the kit. Lane 2 represents the binding flowthrough, and contains the proteins that have been depleted from the serum sample. Figure 2. Depletion of Abundant Proteins. A 2D gel was run after the abundant serum proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. The abundant proteins including albumin, a-antitrypsin, transferin and haptoglobin have been removed from the sample, allowing the visualization and detection of the lower abundance proteins of interest. Next Click for expanded view Kit Specifications Maximum Column Capacity 500 μg Minimum Column Capacity 200 μg Minimum Elution Volume 30 μL Time to Process 10 Samples 30 minutes Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment. ComponentCat. 17300 (25 preps)Wash Solution G125 mLElution Buffer C8 mLProtein Neutralizer4 mLMini Spin Columns25Collection Tubes25Elution Tubes (1.7 mL)25Product Insert1 Documentation PROTOCOLS (17300) ProteoSpin™ Abundant Serum Protein Depletion Kit - Protocol (25 prep) SAFETY DATA SHEETS (17300) ProteoSpin™ Abundant Serum Protein Depletion Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Clinical Proteomics: Liquid Chromatography–Mass Spectrometry (LC–MS) Purification Systems Journal Protein Chromatography. 2016. Authors Henry M, Meleady P. Title Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation Journal The Journal of Clinical Investigation. 2016. Authors Yost CC, Schwertz H, Cody MJ, Wallace JA, Campbell RA, Vieira-de-Abreu A, Araujo CV, Schubert S, Harris ES, Rowley JW, Rondina MT, Fulcher JM, Koening CL, Weyrich AS, Zimmerman GA Title Doublecortin-Like Kinase 1 Is Elevated Serologically in Pancreatic Ductal Adenocarcinoma and Widely Expressed on Circulating Tumor Cells Journal PLoS ONE. 2015. Authors Dongfeng Qu, Jeremy Johnson, Parthasarathy Chandrakesan, Nathaniel Weygant, Randal May, Nicole Aiello, Andrew Rhim, Lichao Zhao, Wei Zheng, Stanley Lightfoot, Shubham Pant, Jeremy Irvan, Russell Postier, James Hocker, Jay S. Hanas, Naushad Ali, Sripathi M. Sureban, Guangyu An, Michael J. Schlosser, Ben Stanger, Courtney W. Houchen Title Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach. Journal Journal of Proteomics. 2013. Authors Kim PY, Tan O, Diakiw S, Carter D, Sekerye EO, Wasinger VC, Liu T, Kavallaris M, Norris MD, Haber M, Chesler L, Dolnikov A, Trahair TN, Cheung NK, Marshall GM, Cheung BB. Title Quantitation of irbesartan and major proteins in human plasma by mass spectrometry with time-of-flight analyzer. Journal Journal of Pharmaceutical and Biomedical Analysis. 2011. Authors Lu CY, Feng CH. Title Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals. Journal Analytical Biochemistry. 2010. Authors Kanekoa H, Kamiieb J, Kawakamib H, Anadac T, Hondac Y, Shiraishid N, Kamakurae S, Terasakif T, Shimauchia H, Suzukic O. Title Modification of major plasma proteins by acrylamide and glycidamide: preliminary screening by nano liquid chromatography with tandem mass spectrometry. Journal Analytica Chimica Acta. 2010. Authors Chia-Hsien Fenga and Chi-Yu Lu. Title Analysis of angiotensin II receptor antagonist and protein markers at microliter level plasma by LC?MS/MS Journal Journal of Pharmaceutical and Biomedical Analysis.49 123?128. 2009. Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ Abundant Serum Protein Depletion Kit For the rapid depletion of abundant proteins from serum samples USD$285.00 SKU 17300 Format Spin Column Size 25 Preps Quantity