Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits USD$749.00 SKU TM38650 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for PSTVd Available in TaqMan format for analysis The Potato Spindle Tuber Viroid (PSTVd) was the first viroid to be identified, and the natural hosts for this viroid are potatoes and tomatoes. Potato spindle tuber disease has been found in the United States, Canada, Russia and South Africa, and it is one of the most destructive diseases of potatoes. PSTVd is a small (359 nucleotide), circular, single-stranded RNA molecule closely related to the Chrysanthemum stunt viroid. The viroid attacks all varieties of potato, is known to spread rapidly and often occurs in combination with virus diseases. Symptoms of PSTVd infection range from mild to severe, with severe infections resulting in colour change in the foliage, smaller leaves, spindle like elongation, and a slower rate of sprouting. There are 2 kits available for the detection of PSTVd: TaqMan PCR Kit Click to expand options PLMVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents PLMVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM38650 (100 preps) Cat. TM38610 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - PSTVd Primer & Probe Mix 280 μL 280 μL PSTVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM38610) PSTVd TaqMan Probe/Primer and Control Set - Protocol (100 rxns) (TM38650) Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Kit - Protocol (100 rxns) POSTERS Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid Related Products Potato Spindle Tuber Viroid (PSTVd) TaqMan Probe/Primer and Control Set Plant RNA/DNA Purification Kit Plant/Fungi Total RNA Purification Kit
Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits USD$749.00 SKU TM38650 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
