NGS Library Quantification Kit (for Small RNA-Seq) PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations For research use only and NOT intended for in vitro diagnostics. Powered by Bioz NGS Library Quantification Kit (for Small RNA-Seq) PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations SMART Save USD$115.00 USD$92.00 SKU 61600 Size 100 Reactions Quantity Overview Details Documentation Citations Overview Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrationsDNA is accurately quantified by using a standard curve constructed from the provided DNA StandardsSpecially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries. Details Supporting Data Previous Figure 1. A representative qPCR baseline graph showing the successful amplification of Quantified NGS Library Standards (Green) with a range from 20 pM to 2 fM, using Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) (Panel A). Duplicate amplification of a sample Small RNA-Seq library (at 1:10,000 dilution) was performed (Orange). The derived library concentration was 9.41 nM. Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) is of good quality as shown with the high PCR efficiency and correlation in the standard curve (Panel B) with low background signals (No Template Control – NTC as Yellow in Panel A). Figure 2. Accurate standard curve quantification. Norgen's NGS Library Quantification Kit (for Small RNA-Seq) was compared head-to-head with two competitor's NGS Library Quantification Kits on a Bio-Rad CFX96 Real-Time PCR system. Norgen's kit showed both high correlation and PCR efficiency when compared to the competitors' kits. Next Click for expanded view Storage Conditions Upon receipt, store Norgen's NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower. ComponentCat. 61600 (100 reactions)2X Real-Time PCR Master Mix1 mLNGS Library Quantification Primer Set Mix600 µLQuantified NGS Library Standards (for Small-RNA Seq)*5 x 80 µL StandardsProduct Insert1* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM Documentation PROTOCOLS (61600) NGS Library Quantification Kit (for Small RNA-Seq) - Protocol (100 rxns) SAFETY DATA SHEETS (61600) NGS Library Quantification Kit (for Small RNA-Seq) - Safety Data Sheet Citations Title Hydrogel-Embedded Poly(Lactic-co-Glycolic Acid) Microspheres for the Delivery of hMSC-Derived Exosomes to Promote Bioactive Annulus Fibrosus Repair Journal SAGE Journals. 2022. Authors Tyler J., Keti Vaso, and James C. Iatridis, et al. Title Alteration of miRNAs in Small Neuron‑Derived Extracellular Vesicles of Alzheimer's Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses Journal Journal of Molecular Neuroscience . 2022. Authors Devrim Yagmur Durur, Bora Tastan, Kemal Ugur Tufekci, Melis Olcum, Hamdiye Uzuner, Gökhan Karakülah, Gorsev Yener, Sermin Genc Related Services NGS Related Products PCR Purification Kit PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) CleanAll DNA/RNA Clean-Up and Concentration Micro Kit
NGS Library Quantification Kit (for Small RNA-Seq) PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations For research use only and NOT intended for in vitro diagnostics. Powered by Bioz NGS Library Quantification Kit (for Small RNA-Seq) PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations SMART Save USD$115.00 USD$92.00 SKU 61600 Size 100 Reactions Quantity
