FFPE RNA/DNA Purification Plus Kit Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FFPE RNA/DNA Purification Plus Kit Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples USD$575.00 SKU 54300 Format Plus Size 50 Preps Quantity Overview Details Documentation Citations Overview Fast and easy processing using rapid spin-column format High yields and quality of nucleic acids Separate fractionation of RNA and DNA Isolate total RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis. Details Supporting Data Previous Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen's FFPE RNA/DNA Purification Plus Kit isolates FFPE RNA and DNA that exceeds the yield of competitors. Total RNA and DNA was isolated from equal amount of hamster FFPE spleen sections (20 micron thickness) using Norgen's FFPE RNA /DNA Purification Plus Kit and a leading competitor’s kits. Triplicate isolations were performed for each product. The top graphs demonstrate the mean yield of RNA (Panel A) and DNA (Panel B) according to NanoDrop measurement. The bottom graphs showed the mean 260:280 ratio and 260:230 ratio of RNA (Panel C) and DNA (Panel D) according to NanoDrop measurement. Norgen's kit consistently purified total RNA and DNA with a higher yield and higher quality than for those obtained using the market competitor's kits. Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen's FFPE RNA/DNA Purification Plus Kit is of high quality with effective qPCR amplification. Panel A showed that in a qPCR using primers against the 5S rRNA gene using equal amount of FFPE DNA as template, the DNA isolated with Norgen's kit showed better amplification (lower Ct value). Moreover, Norgen's FFPE RNA/DNA Purification Plus Kit isolates true total RNA including microRNA. With the same amount of RNA as template in RT-qPCR reactions for transcripts of miR-21 (Panel B for microRNA) and beta-Actin (Panel C for mRNA), RNA isolated by Norgen showed better amplification in beta-Actin and significantly better in miR-21 microRNA. Next Click for expanded view Kit Specifications Maximum Binding Capacity Up to 35 μg RNA Up to 10 μg DNA Maximum Column Loading Volume 600 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material 4 sections <20 μM thick 10 mg of unsectioned block Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year after the date of shipment. ComponentCat. 54300 (50 preps)Digestion Buffer A2 x 25 mLBuffer RL40 mLEnzyme Incubation Buffer A6 mLWash Solution A2 x 38 mLElution Solution A6 mLElution Buffer F15 mLProteinase K2 x 12 mgDNase I1 vialRNA Purification Micro Columns50DNA Purification Micro Columns50Collection Tubes100Elution Tubes (1.7 mL)100Product Insert1 Documentation PROTOCOLS (54300) FFPE RNA/DNA Purification Plus Kit - Protocol (50 prep) SAFETY DATA SHEETS (54300) FFPE RNA/DNA Purification Plus Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Multiregion sequencing of sarcomatoid renal cell carcinoma arising from autosomal dominant polycystic kidney disease Journal Molecular Genetics & Genomic Medicine. 2022. Authors Elizabeth Lee, Peiyong Guan, Abner Herbert Lim, Jui Wan Loh, Grace Fangmin Tan, Tracy Loh, Dave Yong Xiang Ng, Jing Yi Lee, Shane Goh, Wei Liu, Cedric Chuan-Young Ng, Bin Tean Teh, Jason Yongsheng Chan Title Decreased expression of miR-23b is associated with poor survival of endometrial cancer patients Journal Research Square. 2022. Authors Paweł K. Włodarski, Klaudia Klick, Tomasz M. Grzywa, Alicja Klinke, Aleksandra Mielniczuk, Jarosław Wejman, Joanna Ostrowska, Agata Gondek Title Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid Journal Pathol Oncology & Research. 2022. Authors Zaytseva M, Usman N, Salnikova E, Sanakoeva A, Valiakhmetova A, Chervova A, Papusha L, Novichkova G, Druy A Title The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer Journal Cancer Epidemiology, Biomarkers and Prevention. 2017. Authors Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola Title MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma Journal The Laryngoscope. 2015. Authors Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD Title In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA Journal PLOS ONE. 2014. Authors David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin. Journal The Journal of Biological Chemistry. 2014. Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis. Journal Cancer Cell. 2013. Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y. Title Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis. Journal International Journal of Gynecology Pathology. 2012. Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR. Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. Journal BMC Research Notes. 2012. Authors Patnaik SK, Kannisto E, Yendamuri S. Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. Journal PLoS One. 2011. Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S. Related Services NGS RNA Isolation Related Products FFPE RNA/DNA Purification Plus Kit FFPE RNA Purification 96-Well Kit FFPE DNA Purification Kit RNA/DNA/Protein Purification Plus Kit Leukocyte RNA Purification Kit
FFPE RNA/DNA Purification Plus Kit Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz FFPE RNA/DNA Purification Plus Kit Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples USD$575.00 SKU 54300 Format Plus Size 50 Preps Quantity