Norgen’s Endotoxin Removal Kit (Mini) is designed for the rapid removal of endotoxins from up to 40 µg of previously purified DNA. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in downstream applications. With Norgen’s Endotoxin Removal Kit (Mini) endotoxin levels are efficiently reduced to 0.1 EU/µg DNA or less. 

Purification is based on spin-column chromatography using Norgen’s proprietary resin as the separation matrix.  Briefly, the DNA sample is first mixed with the provided Binding Solution, the sample is placed into the top reservoir of the column, and Endotoxin Removal Solution is added.  The column is then spun in order to bind the DNA (BIND).  Under these conditions only the DNA will bind to Norgen's resin while the contaminating endotoxins are removed in the flowthrough.  The bound DNA is then washed to remove any remaining impurities (WASH).  Lastly, the endotoxn-free plasmid DNA is eluted into 50 - 100 µL of the provided Elution Buffer or water (ELUTE).  Please see the procedure flowchart to the right.

The Endotoxin Removal Kit (Mini) can be used to purify plasmids up to 13,000 bp in size, with average recoveries of greater than 90%.  The purified DNA is of the highest quality and can be used in a number of downstream applications including sequencing, cloning and transfection.

FEATURES AND BENEFITS

• Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes
• Endotoxin-free plasmid DNA - Using Norgen’s Endotoxin Removal Kit (Mini), endotoxin levels are reduced to  less than or equal to 0.1 EU/µg plasmid DNA
• High recovery of input DNA - Average DNA recovery is > 90% of the input amount
• Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with sequencing, cloning and transfections