Norgen’s BAC DNA MiniPrep Kit is designed for the rapid preparation of Bacterial Artificial Chromosomes (BACs) and other large DNA constructs from small batch cultures of Escherichia coli. The BAC DNA MiniPrep Kit typically recovers between 0.6 and 1 µg of DNA from 3 mL of bacterial culture. Purification of constructs up to 130 kbp in size have been verified.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix.  Briefly, the bacteria harbouring the plasmid is pelleted and lysed, and the released plasmid DNA is bound to Norgen's column in the presence of Binding Solution (BIND).  Under these conditions only the DNA will bind to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough.  The bound DNA is then washed to remove any remaining impurities (WASH).  Lastly, the purified DNA is eluted into 30 - 50 µL of the provided Elution Buffer or water (ELUTE).  Please see procedure flowchart to the right.  

Norgen's BAC DNA Miniprep Kit purifies DNA without alcohols, a significant improvement over other techniques to achieve full compatibility with alcohol-sensitive applications. The purified DNA is free of genomic DNA, RNA and cellular proteins and is suitable for many downstream applications.

FEATURES AND BENEFITS

• High Recovery - 0.6 to 1 µg of BAC DNA can be purified from 3.0 mL of bacterial culture.
• Alcohol-free protocol - No alcohols are used durig the purification process, thus the DNA is compatible with alcohol sensitive downstream applications.
• Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 1 hour.
• Recovered DNA is suitable for downstream applications -  Purified BAC DNA is fully compatible with restriction enzyme digestions and sequencing.
• High binding capacity of columns - The binding capacity of the columns in the BAC DNA MiniPrep Kit is 25 µg