Norgen’s Low Copy Plasmid MiniPrep Kit is designed for the rapid preparation of low copy number plasmid DNA from small batch cultures of E. coli.  The kit is able to purify 0.5 - 1 µg of low copy number plasmid DNA from 3 mL of bacterial culture.  Purification of plasmids up to 14,000 bp in size have been verified.  

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the bacteria harbouring the plasmid is pelleted and lysed, and the released plasmid DNA is bound to Norgen's column in the presence of Binding Solution (BIND).  Under these conditions only the DNA will bind to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough.  The bound DNA is then washed to remove any remaining impurities (WASH).  Lastly, the purified DNA is eluted into 30 - 50 µL of the provided Elution Buffer or water (ELUTE).  Please see procedure flowchart to the right.  

 The kit purifies DNA without alcohols, a significant improvement over other techniques to achieve full compatibility with alcohol-sensitive applications. The purified DNA is free of genomic DNA, RNA and cellular proteins and is suitable for many downstream applications.  

FEATURES AND BENEFITS

• Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
• High yield - 0.5 to 1 µg of low copy number plasmid DNA can be purified from 3 mL of bacterial culture
• Recovered plasmid DNA is suitable for downstream applications - Purified plasmid DNA is fully compatible with restriction enzyme digestions, and manual or  automated sequencing.
• Alcohol-free protocol - No alcohols are used during the purification process, thus the DNA is compatible with alcohol sensitive downstream applications.