Figure 1.  Consistent Isolation of High Quality Leukocyte RNA.   Norgen’s Leukocyte RNA Purification Kit isolates leukocyte RNA of high quality with great consistency. Total leukocyte RNA was isolated from 100 µL of hamster blood using Norgen’s Leukocyte RNA Purification Kit. A total of 6 replicates were performed, and 7 µL of the 50 µL purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, Norgen's kit not only isolated high and consistent yields of total RNA, but the RNA was also of high quality as evidenced by intactness of the major 28S and 18S rRNA.

Figure 2.  Higher Yield and Quality of Leukocyte RNA Isolated by Norgen's Leukocyte RNA Purification Kit.   Norgen’s Leukocyte RNA Purification Kit isolates Leukocyte RNA that exceeds the yield and quality of competitors. Total RNA was isolated from 200 µL of hamster blood using Norgen’s Leukocyte RNA Purification Kit and a leading competitor’s kit. One microliter of the 50 µL purified RNA was resolved on an Agilent RNA Nano 6000 chip. The gel diagram (Upper panel) and the electropherogram (Lower panel) showed better quality of RNA isolated by Norgen’s kit. In particular, RNA isolated by Norgen’s Leukocyte RNA Purification Kit did not have any evidence of the RNA degradation that was present in RNA isolated by the competitor’s kit (Red Circle). In addition, Norgen’s kit isolated higher amounts of RNA (Blue Line) with the additional recovery of small RNAs including miRNA (Black Circle) which were not present in RNA isolated by the competitor’s kit. The above observation was further confirmed in RT-qPCR reactions as illustrated Figure 3.

Figure 3.  High Yield of a Diversity of RNA Species.   Norgen’s Leukocyte RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA including microRNAs. Total RNA was isolated from 200 µL of hamster blood sample using Norgen’s Leukocyte RNA Purification Kit and a leading competitor’s kit as illustrated in Figure 2. Two hundred nanograms of the purified RNA (both 50 µL elution volumes) was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Lower Panel) and for detecting miR-21(Upper Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen’s kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Upper Panel). Also, Norgen’s kit successfully isolated a similar amount of the large RNA compared to the competitor’s kit (Lower Panel) indicating the full diversity of RNA species isolated.