Norgen’s DNA Gel Extraction Kit enables rapid purification of DNA fragments that have been fractionated on agarose gels.  The kit effectively removes gel contaminants, allows for buffer exchange and yields high quality DNA appropriate for many downstream applications. 

Purification is based on spin column chromatography.  Briefly, the DNA of interest is run on an agarose gel and the band is then excised from the gel.  Binding Solution is added to the gel slice to melt the agarose, and the released DNA is then bound to Norgen's column (BIND).  Under these conditions only the DNA will bind to Norgen's column while the agarose and other impurities are removed in the flowthrough.  The bound DNA is then washed to remove any remaining impurities (WASH).  Lastly, the purified DNA is eluted into 30 - 50 µL of the provided Elution Buffer or water (ELUTE).  Please see the procedure flowchart to the right.

Norgen’s DNA Gel Extraction Kit effectively removes gel contaminants, allows for buffer exchange and yields high quality DNA appropriate for many downstream applications. The kit is able to recover DNA ranging in size from 100 bp to 15,000 bp. Each column has a binding capacity of 10 µg, and the typical purification yield is up to 90% of the input amount.  

FEATURES AND BENEFITS

• Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes.
• High Recovery - Recovery of DNA fragments up to 90% for inputs of > 1 mg.
• Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with restriction digestions, ligations, sequencing, PCR and other downstream applications.
• Useable with a variety of agarose percentages - Kit is compatible with agarose gel concentrations of up to 4%.