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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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Blood Genomic DNA Isolation Micro Kit
(Cat# 18200)
For the rapid preparation of high quality DNA from whole blood, buffy coat, plasma and serum
- Input volumes up to 150 µL
- No phenol required for DNA isolation
- Extract DNA from whole blood, buffy coat, plasma and serum
- Fast and convenient spin column protocol
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Norgen’s Blood Genomic DNA Isolation Micro Kit is designed for the rapid preparation of genomic DNA from up to 1500 µL of whole blood, buffy coat, plasma and serum.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the sample is lysed and the released genomic DNA is bound to Norgen's column in the presence of Binding Solution (BIND). Under these conditions only the genomic DNA is bound to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 100 - 200 µL of the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.
The Blood Genomic DNA Isolation Micro Kit allows for the isolation of genomic DNA from the blood of various species, including humans. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 50 preparations. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including PCR and Southern Blot analysis. FEATURES AND BENEFITS
- No Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals
- Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
- Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood, buffy coat and serum
- Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis.
- High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Micro Kit is observed.
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| APPLICATIONS
Genomic DNA isolated using the Blood Genomic DNA Isolation Micro Kit is well-suited to a wide range of downstream applications including:
- qPCR
- PCR
- Sequencing
- Restriction Digests
- Southern blotting
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Figure 1. High Yields of Genomic DNA Isolated from Whole Blood. Genomic DNA was isolated from two different 150 µL whole blood samples using Norgen's Blood Genomic DNA Isolation Micro Kit and a leading competitor's kit. Following isolation, 10 µL from 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood Genomic DNA Isolation Micro Kit demonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgen’s HighRanger 1kb DNA Ladder.
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Figure 2. Purified DNA Can be Amplified in a Real-time PCR (SYBR Green) Reaction. Genomic DNA was isolated from 150 µL of whole rat blood ( Δ ) and whole rabbit blood (O) samples using Norgen's Blood Genomic DNA Isolation Micro Kit (Blue) and a leading competitor's kit (Red). Two µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with 5s rDNA primers. The real-time PCR was successful in amplifying 5s gene from both the rat and rabbit blood DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield amd purity of DNA isolated using Norgen's kit. |
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Kit Specification
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Details
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Maximum blood input
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150 µL
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| Average Yield (150 µL of blood) |
3 to 5 µg*
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| Time to complete 10 purifications |
30 minutes |
* Yield will vary depending on the type of blood processed
Blood Genomic DNA Isolation Kit Contents
1. Resuspension Solution
2. Lysis Solution
3. Proteinase K (lyophilized)
4. Wash Solution I
5. Wash Solution II
6. Elution Buffer
7. Micro Spin Columns
8. Collection Tubes
9. Elution Tubes
10. Product Insert
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Blood Genomic DNA Isolation Kit |
18200 |
50 preps |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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