Norgen’s Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and filamentous fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit.

The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples. Briefly, the cells are macerated using the provided Lysis Solution, and the released DNA is bound to Norgen's column in the presence of Binding Solution (BIND). Under these conditions only the DNA will bind to the resin while most of the contaminating RNA and proteins are removed in the flowthrough.  The bound DNA is then washed to remove any remaining impurities (WASH).  Lastly, the purified DNA is eluted into 50-100 µL of the provided Elution Buffer or water (ELUTE).  Please see the procedure flowchart to the right.

The purified DNA is of the highest integrity, and can be used in a number of downstream applications including real - time PCR, SNP, Southern blotting and sequencing.

FEATURES AND BENEFITS

• No liquid nitrogen required for homogenization - Liquid nitrogen is not required for homogenization of samples, making DNA purification rapid and convenient.
• Isolate DNA from a wide range of samples - DNA can be isolated from a wide range of plant and filamentous fungi samples.
• Isolate total DNA, including viral DNA - Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA.
• Rapid and simple processing - Rapid spin-column format allows for the processing of multiple samples in 45 minutes.
• High yield of DNA - High yields of purified DNA can be isolated with this kit.
• No phenol :chloroform extractions - DNA is isolated without the use of harmful chemicals such as phenol or chloroform. The DNA is of the highest quality and can be used in a number of downstream applications.