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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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PCR Purification Kit
(Cat# 14400, 24800, 45700)
For the rapid purification of amplified DNA products from PCR mixes
- Purify amplified DNA ranging from 100 bp -15,000bp in size
- Fast and efficient spin column format
- Available in a 50 prep size and a 250 prep size
- Also available in 96 well format
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Norgen’s PCR Purification Kit enables rapid purification of amplified DNA products from PCR mixes. The kit is able to purify amplified DNA ranging in size from 100 bp to 15,000 bp, and the typical purification yield is greater than 90% of the input amount. The kit removes all primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product.
Purification is based on spin-column chromatography. Briefly, the PCR product is first mixed with the provided Binding Solution, and the DNA is then bound to Norgen's column (BIND). Under these conditions only the DNA will bind to Norgen's column while the primers, dimers and other contaminants are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified PCR product is eluted into 30 - 50 µL of the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
FEATURES AND BENEFITS
- Fast and easy processing - Rapid spin-column format processes 10 samples in 15 minutes, while a 96-well plate can be processed in 20 minutes.
- Purification from all PCR by-products - Remove all primer-dimers, primers, mineral oil, unicorporated nucleotides, enzymes and salts.
- High recovery - Recovery of greater than 90% of input amount.
- Can be used as an alternative to organic extraction and ethanol precipitation - This kit can also be used to clean-up various enzymatic reactions including digestions and Klenow reactions.
- Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with restriction digestions, ligations, labeling, PCR, and sequencing.
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| APPLICATIONS
DNA purified using Norgen's PCR Purification Kit can be used in a number of downstream applications including:
- Restriction digestions
- Ligations
- Labeling
- PCR
- Sequencing
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Figure 1. High Yield Purification. The high yield of Norgen’s PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor. The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1. Norgen’s kit shows a significantly higher recovery than the competitor’s kit.
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Figure 2. Removal of Primer-Dimers. Primer-dimers present in PCR reactions are effectively removed during the purification process with Norgen’s PCR Purification Kit. One microgram of a 700 bp PCR fragment, spiked with 250 pmole of primer (Lane 1) was purified using Norgen’s kit, with high recovery and no traces of spiked primer present in the elutions (Lanes 2 and 3). Lane 1 contains 400 ng (40%) of the input, and Lanes 2 and 3 contain 20 µL (40%) of the 50 µL elution (duplicate). Lane M is Norgen’s MidRanger 1kb DNA Ladder. Eluted DNA was resolved on a 1X TAE, 1% agarose gel. |
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Kit Specifications - Spin Column
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Column Binding Capacity
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10 µg
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Average Primer Removal
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> 90%
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Size of DNA Purified
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100 - 15,000 bp
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Minimum Elution Volume
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30 µL
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Average Recovery
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> 90%
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Time to Complete 10 Purifications
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15 minutes
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PCR Purification Kit Contents - Spin Columns
1. Binding Solution
2. Wash Solution
3. Elution Buffer
4. Spin Columns inserted in Collection Tubes
5. Elution Tubes
6. Product Insert
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Kit Specifications
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Binding Capacity Per Well
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15 ug
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Maximum Loading Volume Per Well
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500 uL
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Size of DNA Purified
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100 – 15,000 bp
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Average DNA Recovery
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> 90%
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Average Primer Removal
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> 90%
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Minimum Elution Volume
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50 uL
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Time to Complete 96 Purifications
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20 minutes
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PCR Purification Kit Contents - 96-Well Plates
1. Binding Solution
2. Wash Solution
3. Elution Buffer
4. 96-Well Filter Plate
5. Adhesive Tape
6. 96-Well Collection Plate
7. 96-Well Elution Plate
8. Product Insert
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
PCR Purification Kit |
14400 |
50 preps |
Buy Now |
PCR Purification 96-Well Kit |
24800 |
192 preps |
Buy Now |
PCR Purification Kit |
45700 |
250 preps |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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