The ProteoSpin™ Endotoxin Removal Kit efficiently reduces endotoxin levels to ≤ 0.01 EU/µg of protein using spin column chromatography.   This kit works by binding proteins to the spin column matrix while endotoxins and other contaminants are washed away.  The purified proteins and peptides are then eluted and ready for downstream applications.  A uniqued feature of this kit is that the endotoxins can be washed away repeatedly until desirable levels of endotoxins are obtained.  Other commercially available kits employ membranes to bind the endotoxins and therefore can quickly become saturated with endotoxin, however  Norgen's columns do not become saturated as the endotoxins are not bound on the column.  In addition, no further purification steps are required to clean-up the sample prior to use in downstream applications.  Norgen's kit will desalt, remove detergents and allow for buffer exhange while simultaneously removing endotoxins, therefore saving considerable time and expense. The purified protein is suitable for in vivo and in vitro introduction into cells and organisms, monoclonal and polyclonal antibody generation, and other applications such as mass spectrometry, gel electrophoresis, immunoassays and Western blots.

FEATURES AND BENEFITS

• Endotoxin-free proteins and peptides - The ProteoSpin™ Endotoxin Removal Maxi Kit reduces endotoxin levels to less than 0.1 EU/µg of protein.
• Fast and easy processing - Efficient processing of multiple samples in 20 to 30 minutes using an easy-to-use protocol.
• High recovery - Protein recovery is over 95% of input.
• No further clean-up of protein samples required - Norgen's kit provides desalting, buffer exchange and detergent removal while removing endotoxins
• Recovered protein is suitable for downstream applications - Purified protein is suitable for in vivo and in vitro introduction into cells and organisms, monoclonal and polyclonal antibody generation, mass spectrometry, gel electrophoresis, immunoassays, and Western blots
• Columns are ready-to-use - Unlike other endotoxin removal methods, there is no need for lengthy column or resin preparation
• No endotoxin carry-over - No column regeneration, unlike other gel-based endotoxin removal procedures, which can lead to endotoxin carry-over.  Columns are single use only.