Input Volume	Output Volume	Concentration Factor
20 mL	4 mL	5X
60 mL	4 mL	15X
100 mL	4 mL	25X

To demonstrate the ability of the ProteoSpin^TM CBED Maxi Kit to concentrate protein samples, 8 mg of BSA was spiked into 20, 60 and 100 mL of a 50 mM sodium acetate solution (pH 4.5).  The samples were processed as per the provided protocol, and all samples were eluted into 4 mL of the provided Elution Buffer.  The 20 mL input was therefore concentrated 5X, the 60 mL input was concentrated 15X, and the 100 mL input was concentrated 25X.

Input         Concentration	Output          Concentration	Concentration Factor
8 mg / 20 mL	7.89 mg / 4 mL	5X
(0.4 mg/mL)	(1.97 mg/mL)

To further demonstrate the ability of the ProteoSpin^TM CBED Maxi Kit to concentrate protein samples, 8 mg of BSA was spiked into 20 mL of a 50 mM sodium acetate solution (pH 4.5).  The sample was processed as per the provided protocol, and was eluted into 4 mL of the provided Elution Buffer.  The amount of recovered protein was then determined using the BioRad Protein Assay.  It was found that 7.89 mg of the input protein was recovered in 4 mL, corresponding to a 5X protein concentration based on mass.

 Figure 1.  High Protein Recovery. Increasing amounts of BSA (1, 2, 4, 6 and 8 mg) were spiked into 20 mL of a 50 mM sodium acetate solution, and were concentrated using the ProteoSpinTM CBED Maxi Kit.  The provided protocol was followed, and the concentrated BSA was eluted into 2 separate 4 mL elutions using the provided Elution Buffer. The elutions were then combined, and the amount of protein recovered in each elution was determined using the BioRad Protein Assay.  The ProteoSpin™ CBED Maxi Kit allows for high protein recoveries of up to 90%.