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Item |
Cat. No. |
Kit Size |
Price (USD) |
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| RNA/Protein Purification Kit |
23000 |
20 |
$165.00 |
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Figure 1. Simultaneous Isolation of RNA and Proteins from HeLa cells, yeast cells and E. coli
Total RNA and total proteins were isolated simultaneously from 10^6 HeLa cells, 5 x 10^6 yeast cells and 5 x 10^7 E. coli cells using Norgen’s kit. Panel A is a 1X MOPS 1.5% agarose gel showing the total RNA. Lane M is Norgen’s 1 kb RNA Ladder, Lanes 1 and 2 contain 10 µL of the elution from the HeLa cells, Lanes 3 and 4 contain 3 µL of the elutions for the yeast cells, and Lanes 5 and 6 contain 3 µL of the elutions for the E. coli cells. Panel B is a 10% SDS-PAGE gel containing the total proteins that were isolated. In each case, 25 µL out of the elutions was loaded. Lanes 1 and 2 contain the HeLa proteins, Lanes 3 and 4 contain the yeast proteins and Lanes 5 and 6 contain the E. coli proteins. The RNA and proteins are intact and of the highest quality, and can be used in a number of different downstream applications.
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User-Friendly Procedure (click to enlarge)
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More Information
Norgen’s RNA/Protein Purification Kit provides a rapid method for the isolation and purification of total RNA and total proteins simultaneously from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The simultaneous isolation of the RNA and proteins from the same sample not only saves time, but will also be of a great benefit when isolating RNA and proteins from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and proteins are derived from the same sample, therefore eliminating inconsistent results and variability. In addition, the kit can also be used for the simultaneous isolation of genomic DNA and proteins from a single sample.
With Norgen’s RNA/Protein Purification Kit, the purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus the RNA will bind to the column while the proteins are removed in the flowthrough. After the RNA is washed and eluted from the column, the column is then regenerated and used to purify the proteins that are present in the flowthrough. Norgen’s kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA is of the highest integrity, and can be used in a number of downstream applications. The purified proteins can also be used in a number of different downstream applications.
RNA/Protein Purification Kit - Benefits
- Reduce variablility - RNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variablility.
- Isolate from small samples - Simultaneous isolation of RNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
- Complete purification - The RNA and proteins are all column purified.
- Rapid procedure - Isolate total RNA and proteins from a single sample in less than 40 minutes.
- Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA.
- Process a wide range of sample types - Isolate total RNA and proteins from cultured animal cells, tissue, blood, bacteria, yeast, fungi or plants.
Applications
RNA
- Real time PCR
- Reverse transcription PCR
- Northern blotting
- RNAse protection
- Primer extension
- Expression array analysis
Protein
- SDS-PAGE analysis
- Western blots
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