Saliva RNA Collection and Preservation Devices For simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva RNA Collection and Preservation Devices For simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature SKU RU53800 Size 50 Devices Contact Distributor Overview Details Documentation FAQs Citations Overview Reliable and cost-effective Non-invasive, user-friendly sample collection and preservation in one convenient kit Preserved RNA is stable for up to 2 months at room temperature Compatible with most RNA isolation methods including Norgen’s Saliva/Swab RNA Purification Kits High quality RNA is suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing and microarray Inactivates viruses including SARS-CoV-2 upon mixing - Explore the Application Note Shipping accessories can be purchased separately CE-IVD marked version available Norgen’s Saliva RNA Collection and Preservation Devices are designed for simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components: Saliva Collection Funnel and Collection Tube Collection Tube Cap Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies. Saliva RNA Preservative Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature. Nucleic Acid Isolation from Preservative Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Details Supporting Data Previous Figure 1. Stability of RNA Preserved in Norgen's Saliva RNA Preservative at room temperature for up to 2 months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva RNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 2 months. Saliva RNA was subsequently isolated at day 0, day 4, day 6, day 16, 1 month and 2 months from 0.25 mL of the saliva/preservative sample using supplementary protocol associated with Norgen's Total RNA Purification Kit (Cat. 17200). For visual analysis, 7.5 µL from 50 µL elution was mixed with RNA loading dye and was run on a 1x MOPS agarose gel. RNA integrity was maintained after the saliva samples were stored up to 2 months at ambient temperatures in Norgen’s Saliva RNA Preservative. Figure 2. Stability of three house keeping genes over a 2 month period. GAPDH, Human 18S rRNA and beta actin genes were monitored from saliva samples stored at ambient temperature with Norgen's Saliva RNA Preservative. RNA was isolated from 250 µL of preserved samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgen's Total RNA Purification Kit (Cat. 17200). Next, 4 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad). All of the genes were preserved and stable over 2 month period. Figure 3. Norgen’s Saliva RNA Preservative is able to successfully preserve saliva RNA samples when used outside of the ideal range of 1:1 at room temperature for 3 days. As the amount of saliva added to 2 mL of Saliva RNA Preservative increased from 0.2 mL to 3 mL, RNase protein and β-actin gene were monitored for RNA stability using one-step RT-PCR. The results indicated that the variation of the saliva input volumes did not significantly influence the RNA gene detection. Figure 4. Detection of Viral, Bacterial and Human RNA from Preserved Saliva Samples. Saliva (2 mL) collected from a single healthy donor was mixed with 2 mL of Saliva RNA Preservative. Norgen’s COVID-19 positive control from the 2019-nCoV TaqMan RT-PCR Kit (E/RdRP gene) (Cat. TM67200) was spiked into the preserved saliva along with HeLa cells (1.6 m cells /ml ) to generate a contrived sample. From the contrived sample, total RNA was purified using Norgen’s Total RNA Purification Kit (Cat. 17200) and 5 genes (16s rRNA, COVID-19 E gene, RdRP-P1 and P2 gene and RP) were analyzed by PCR or RT-PCR to detect viral, bacterial and human genes. Next Click for expanded view Kit Specifications Volume of Saliva Collected 2 mL Volume of Saliva-Preservative Mix 4 mL Preservation Temperature Room Temperature Preservation time Up to 2 months at room temperature Shelf Life and Handling When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date. Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature. The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable. Kit Components Cat. RU53800 (50 Devices) Individual Saliva RNA Collection and Preservation Devices 50 Donor Procedure Flowchart 1 Product Insert 1 Individual Saliva RNA Collection and Preservation Device Contents Saliva Collection Funnel and Collection Tube 1 Collection Tube Cap 1 Preservative Ampoule 1 Donor Instructions 1 Documentation PROTOCOLS (RU53800) Saliva RNA Collection and Preservation Devices - Protocol (50 devices) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - Total RNA Purification from Saliva RNA Collection and Preservation Device Supplementary Protocol - Purification of Viral RNA with the Maxwell® RSC Viral Total Nucleic Acid Purification Kit APPLICATION NOTES Viral Inactivation Using Norgen’s Total Nucleic Acid Preservative and Norgen’s Saliva RNA Preservative Inactivation of Coronavirus in Samples using Norgen's Saliva RNA Preservative FLYERS Customization Flyer Saliva Collection and Preservation Devices FAQs What are the volume and dimensions of each saliva tube? Each saliva tube is a 5 mL tube with dimensions as follows: 65 mm (height) x 16.5 mm (diameter). What is the material of the saliva collection tube? Tube material is as follows: Cap: Polyethylene Tube: Polypropylene (PP) Funnel: Polyphenylene ether Polystyrene (PPE-PS) thermoplastic Can the preserved saliva be frozen to extend the storage period for more than 2 months? Yes, the preserved saliva can be stored at -80 °C for longer periods to preserve RNA. However, Norgen's preservative might not protect the RNA from damage caused due to freeze-thaw of the sample. Citations Powered by Bioz See more details on Bioz Related Products Saliva/Swab RNA Purification Kit Saliva/Swab RNA Purification 96-Well Kit Total Nucleic Acid Preservation Tubes Total RNA Purification Kit
Saliva RNA Collection and Preservation Devices For simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saliva RNA Collection and Preservation Devices For simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature SKU RU53800 Size 50 Devices Contact Distributor