RNA/Protein Purification Plus Kit For sequential isolation of total RNA and total proteins from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/Protein Purification Plus Kit For sequential isolation of total RNA and total proteins from the same sample USD$619.00 SKU 48200 Format Plus Size 50 Preps Quantity Overview Details Documentation Citations Overview Sequentially purify total RNA and total proteins from a single sample Kit includes a gDNA elimination column No sample splitting required No phenol step required for efficient isolation Ideal for small or difficult to obtain samples Purify RNA and proteins from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants Rapid and efficient spin column procedure Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column. Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays. This kit eliminates DNA efficiently using a gDNA removal column. Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and ELISA. The proteins will not require precipitation, resuspending of pellets, or any further cleaning. This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications. Protocol Details Supporting Data Previous Figure 1. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 10 mg hamster liver using Norgen's RNA/Protein Purification Plus Kit. Norgen's RNA/Protein Purification Plus Kit isolated both large RNA (represented by 28S and 18S rRNA) as well as small RNA with high integrity and without having to perform any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, the Bioanalyzer showed that Norgen's RNA/Protein Purification Plus Kit has the added benefit of recovering small RNA. One microgram of the RNA was used in RT-qPCR reactions for the detection of human beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated using Norgen's RNA/Protein Purification Plus Kit showed superior recovery of both large RNA and small RNA including microRNAs as depicted by the successful miR-21 RT-qPCR (Panel C). Figure 2. High Quality Total Proteins Eluted in Mass Spec-Compatible Buffer. Norgen's RNA/Protein Purification Plus Kit provides a column purification step for effective concentration and clean-up of the isolated proteins. The proteins are eluted into a buffer which is compatible with many downstream applications including mass spectrometry as well as standard protein quantification methods (including Bradford assays). In contrast, most competing multiple analyte isolation products require protein precipitation and the precipitated proteins are required to be resuspended in buffer with high-detergent content (such as SDS-PAGE loading dye) for full recovery. In this figure, the protein fraction isolated from hamster liver using Norgen's RNA/Protein Purification Plus Kit was resolved direclty on a 12% SDS-PAGE protein gel. Norgen's RNA/Protein Purification Plus Kit purified the proteins by column and the eluted proteins are already in buffer compatible with downstream applications. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg for RNA Maximum Column Binding Capacity 200 μg for Protein Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Time to Complete 10 Purifications 30 minutes Average Yields*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg) 10-15 μg RNA 70-100 μg protein 30-35 μg RNA 100-150 μg protein * Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage. Storage Conditions and Product Stability The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 48200 (50 preps) Buffer SKP 40 mL Wash Solution A 38 mL Elution Solution A 6 mL Wash Solution C 30 mL Binding Buffer A 8 mL Elution Buffer C 4 mL Protein Neutralizer 4 mL Protein Loading Dye 2 mL gDNA Removal Columns 50 RNA/Protein Purification Columns 50 Collection Tubes 150 Elution Tubes (1.7 mL) 100 Product Insert 1 Documentation PROTOCOLS (48200) RNA/Protein Purification Plus Kit - Protocol (50 prep) SAFETY DATA SHEETS (48200) RNA/Protein Purification Plus Kit - Safety Data Sheet POSTERS FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Artificial gravity partially protects space-induced neurological deficits in Drosophila melanogaster Journal Cell Reports. 2022 Authors Siddhita D. Mhatre, Janani Iyer, Juli Petereit, Roberta M. Dolling-Boreham, Anastasia Tyryshkina, Amber M. Paul, Rachel Gilbert, Matthew Jensen, Rebekah J. Woolsey, Sulekha Anand, Marianne B. Sowa, David R. 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RNA/Protein Purification Plus Kit For sequential isolation of total RNA and total proteins from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/Protein Purification Plus Kit For sequential isolation of total RNA and total proteins from the same sample USD$619.00 SKU 48200 Format Plus Size 50 Preps Quantity