Listeria monocytogenes TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Listeria monocytogenes TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM30450 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Listeria monocytogenes CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis. Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible. TaqMan PCR Kit Click to expand options Listeria monocytogenes TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM30450 (100 preps) Cat. TM30410 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Listeria monocytogenes Primer & Probe Mix 280 μL 280 μL Listeria monocytogenes Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM30450) Listeria monocytogenes TaqMan PCR Kit - Protocol (100 rxns) (TM30410) Listeria monocytogenes TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Listeria monocytogenes TaqMan PCR Kit Dx Listeria monocytogenes TaqMan Probe/Primer and Control Set Milk Bacterial DNA Isolation Kit Food DNA Isolation Kit
Listeria monocytogenes TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Listeria monocytogenes TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM30450 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity